is selected by interactions with the intron’s terminal nucleotide, whereas the other splice site is selected by an extensive network of tertiary interactions.

During splicing, the spliceosome assembles on the pre-mRNA molecule and brings together the donor site at the 5' end of the intron and the acceptor site at the 3' end of the intron. The intron is then ...

Pre-mRNA splicing in a subset of human short introns is governed by a distinct mechanism involving a new splicing factor Protein-coding genes carry the blueprint for protein production. In higher ...

For the splice site at the beginning of an intron--known as its 5' ["five-prime"] splice site, Krainer says, "we know that at the first and second [DNA-letter] position, mutations have a very ...

In the first step of spliceosome assembly, an snRNA molecule known as U1 snRNA binds to the 5' splice site at the beginning of the intron. Until now, it had been thought that the binding strength ...

Group 1 introns are attacked at the 5’ splice site by a nucleotide cofactor, which may be free in the biological milieu or a part of the intron itself, leading to the 3’OH of the adjacent exon ...

the researchers obtained the structure of the U11 snRNP complex and revealed how it recognises the "5' splice site" -- the starting point of an intron -- to initiate the editing process at this ...

This mutation involves the deletion of an adenine in intron 11 that abolishes the canonical splice acceptor site of exon 12 (Perrone et al., 2018). This leads to exon 12 skipping and a frameshift ...

Splicing is the ... an acceptor site, or neither. "Our algorithm attempts to recognize these donor/acceptor sites in pairs, just as a spliceosome 'molecular machine' does in the cell when it cuts out ...

TDP-43 prevents mis-splicing of STMN2 by blocking a cryptic-exon splice site. Antisense oligonucleotides that bind near the site do the same. The ASOs restored full-length STMN2 in TDP-43 mutant motor ...