PCR products were cloned into the pCR4-TOPO plasmid vector using TOPO-TA cloning kits (Invitrogen, Life technologies), transformed into One shot TOP10 E. coli cells (Invitrogen, Life technologies) ...

Approximately 4 μl of each purified SSU rRNA gene product was cloned into a pCR4-TOPO vector using a TOPO TA cloning kit for sequencing (Invitrogen) and transformed by chemical transformation into ...