Serine-arginine (SR) proteins are general metazoan splicing factors that contain an essential arginine/serine-rich (RS) domain. On typical U2-type introns, RS domains contact the branchpoint and 5 ...

is selected by interactions with the intron’s terminal nucleotide, whereas the other splice site is selected by an extensive network of tertiary interactions.

During splicing, the spliceosome assembles on the pre-mRNA molecule and brings together the donor site at the 5' end of the intron and the acceptor site at the 3' end of the intron. The intron is then ...

Pre-mRNA splicing in a subset of human short introns is governed by a distinct mechanism involving a new splicing factor Protein-coding genes carry the blueprint for protein production. In higher ...

For the splice site at the beginning of an intron--known as its 5' ["five-prime"] splice site, Krainer says, "we know that at the first and second [DNA-letter] position, mutations have a very ...

the researchers obtained the structure of the U11 snRNP complex and revealed how it recognises the "5' splice site" -- the starting point of an intron -- to initiate the editing process at this ...

In the first step of spliceosome assembly, an snRNA molecule known as U1 snRNA binds to the 5' splice site at the beginning of the intron. Until now, it had been thought that the binding strength ...

This mutation involves the deletion of an adenine in intron 11 that abolishes the canonical splice acceptor site of exon 12 (Perrone et al., 2018). This leads to exon 12 skipping and a frameshift ...

intron retention; alternative 3’ splice site; and alternative 5’ splice site. The functional consequences on protein isoforms were predictable at numerous AS events. A great divergent co ...

TDP-43 prevents mis-splicing of STMN2 by blocking a cryptic-exon splice site. Antisense oligonucleotides that bind near the site do the same. The ASOs restored full-length STMN2 in TDP-43 mutant motor ...